Workshop on fundamentals and applications of the flow cytometry
Faria Khan, NaMeS PhD student in the environmental chemistry group of IPC PAS took part in the workshop on fundamentals and applications of the flow cytometry in Prince Felipe Research Centre Fundation . Through this 2 weeks training and experimental section, Faria learned the basic concept, general applications and current instrumentation of flow cytometry. The components and systems of current flow cytometers and their historical development were be explained in detail, as well as the conceptual basis of fluorescence and the usefulness of fluorescent reagents, essential for the vast majority of cytometry applications. The training included the lecture-part whereby basics of flow cytometry in accordance with the application of functional assays was discussed. Second part included the flow cell analysis of samples whereby the samples from Faria’s current PhD project were run to evaluate for many functional assays including apoptosis/ necrosis, TMRM, oxidative and mitochondrial stress studies.
Oct. 20 – Nov. 2, 2019
Prince Felipe Research Centre Fundation