Secondment to the CNRS
Nabila Yasmeen, NaMeS Ph.D. student, took part in the research secondment at the Laboratory of Physical Chemistry and Microbiology for Materials and the Environment (LCPME), CNRS in France, under the supervision of Dr. Mathieu Etienne. Nabila has worked on the fabrication of the chemosensor for E. coli strains. This project's main objective For this study, initially, the nonpathogenic, i.e., E. coli k12 strain, was used as the template. 2-Aminophenyl boronic acid was selected as a functional monomer. The basis of this monomer's selection was based on the fact that the hydroxyl groups of boronic acid can react and form a covalent bond with cis-diols of the negatively charged glycoproteins present on the bacteria surface. The electrochemical polymerization was used for MIP synthesis and its characterization. The fabricated MIP based chemosensor can determine the E. coli k12 strain up to 107 cells mL-1. Furthermore, Nabila Yasmeen studied the role of bacterial cell surface alterations, such as bacterial adhesion and fimbriae, over MIP binding. An initial study with three available bacteria strains was performed to check the selectivity of the sensing system. Now, the future work plan is to increase the sensitivity of the prepared sensor.
6 Dec.2019 - 12 Dec. 2020